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adipocyte-conditioned media  (Unilever Plc)

 
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    Unilever Plc adipocyte-conditioned media
    Adipocyte Conditioned Media, supplied by Unilever Plc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adipocyte-conditioned media/product/Unilever Plc
    Average 90 stars, based on 1 article reviews
    adipocyte-conditioned media - by Bioz Stars, 2026-03
    90/100 stars

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    FIGURE 7 | LsAg treatment improves CD4+ T cell inflammation in an adiponectin dependent manner. (A) Schematic diagram of LsAg treatment in obese mice. (B,C) Frequencies of IFN-γ + (B) and IL-17+ (C) CD4+ T cells in the adipose tissue of PBS and LsAg treated mice (pooled data from n = 2 experiments, 3–4 mice each). (D,E) CD4+ T cells positive for IFN-γ (D) and IL-17 (E) from high fat diet (HFD) mice cultured in the presence of adipocyte <t>conditioned</t> media from PBS and LsAg treated mice. (F,G) Plasma and adipocyte conditioned media adiponectin levels in PBS and LsAg-administered HFD mice. (H,I) Frequencies of CD4+ T cells from HFD mice after co-culture with anti-adiponectin (anti-ADPN) antibody and isotype control treated adipocyte conditioned media from LsAg treated mice (pooled data from n = 3 experiments, 2–3 conditions each). Two-tailed non-parametric Mann–Whitney U-test was performed for statistical analysis.
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    FIGURE 7 | LsAg treatment improves CD4+ T cell inflammation in an adiponectin dependent manner. (A) Schematic diagram of LsAg treatment in obese mice. (B,C) Frequencies of IFN-γ + (B) and IL-17+ (C) CD4+ T cells in the adipose tissue of PBS and LsAg treated mice (pooled data from n = 2 experiments, 3–4 mice each). (D,E) CD4+ T cells positive for IFN-γ (D) and IL-17 (E) from high fat diet (HFD) mice cultured in the presence of adipocyte conditioned media from PBS and LsAg treated mice. (F,G) Plasma and adipocyte conditioned media adiponectin levels in PBS and LsAg-administered HFD mice. (H,I) Frequencies of CD4+ T cells from HFD mice after co-culture with anti-adiponectin (anti-ADPN) antibody and isotype control treated adipocyte conditioned media from LsAg treated mice (pooled data from n = 3 experiments, 2–3 conditions each). Two-tailed non-parametric Mann–Whitney U-test was performed for statistical analysis.

    Journal: Frontiers in immunology

    Article Title: Adiponectin Limits IFN-γ and IL-17 Producing CD4 T Cells in Obesity by Restraining Cell Intrinsic Glycolysis.

    doi: 10.3389/fimmu.2019.02555

    Figure Lengend Snippet: FIGURE 7 | LsAg treatment improves CD4+ T cell inflammation in an adiponectin dependent manner. (A) Schematic diagram of LsAg treatment in obese mice. (B,C) Frequencies of IFN-γ + (B) and IL-17+ (C) CD4+ T cells in the adipose tissue of PBS and LsAg treated mice (pooled data from n = 2 experiments, 3–4 mice each). (D,E) CD4+ T cells positive for IFN-γ (D) and IL-17 (E) from high fat diet (HFD) mice cultured in the presence of adipocyte conditioned media from PBS and LsAg treated mice. (F,G) Plasma and adipocyte conditioned media adiponectin levels in PBS and LsAg-administered HFD mice. (H,I) Frequencies of CD4+ T cells from HFD mice after co-culture with anti-adiponectin (anti-ADPN) antibody and isotype control treated adipocyte conditioned media from LsAg treated mice (pooled data from n = 3 experiments, 2–3 conditions each). Two-tailed non-parametric Mann–Whitney U-test was performed for statistical analysis.

    Article Snippet: For adiponectin neutralization experiments, adipocyte conditioned media was treated overnight with 10μg/ml of adiponectinneutralizing antibody (anti-adiponectin, AF1119, anti-mAcrp30; R&D Systems, Minneapolis, MN., USA) or isotype control antigoat IgG (10μg/ml; R&D Systems) (34).

    Techniques: Cell Culture, Clinical Proteomics, Co-Culture Assay, Control, Two Tailed Test, MANN-WHITNEY